Appendix
47
Basic Lab Calculations
BM327 Microbiology Semester 2 Lab Manual
Preface
Introduction
1
Overall Aims and Competencies
2
Schedule of Experimental Tasks
3
Assessment
4
Lab Safety
5
Good Laboratory Technique
6
Aseptic Technique
7
Using a Microscope
Laboratory 1 - Basic Microbiology Skills
8
Lab 1 Aims and Overview
9
Axenic Culture
10
Serial Dilutions
11
Working with Bacterial Plasmids
12
Lab 1 Protocols
13
Lab 1 - Data Analysis
14
Additional Resources/Further Reading for Lab 1
Laboratory 2 - Microbial Identification
15
Lab 2 Aims and Overview
16
Microbial Identification
17
Selective and Differential Media
18
Differential Stains - Gram Staining
19
Moulds and Fungi
20
Lab 2 Protocols
21
Lab 2 - Data Analysis
22
Additional Resources/Further Reading for Lab 2
Laboratory 3 - Pathogens of the Intestinal Tract
23
Lab 3 Aims and Overview
24
Identification of Intestinal Pathogens
25
More Examples of Selective and Differential Media
26
API-20E Tests
27
Biochemical tests: Oxidase and Catalase
28
Oxygen and Microbial Growth
29
Lab 3 Protocols
30
Lab 3 - Data Analysis
31
Additional Resources/Further Reading for Lab 3
Laboratory 4 - Pathogens of the Circulatory System and Skin
32
Aims
33
Identification of staphylococci
34
Biochemical assays: lysostaphin and coagulase
35
MLVA typing
36
Antibiotic Resistance Assays
37
Lab 4 Protocols
38
Lab 4 - Data Analysis
39
Additional Resources/Further Reading for Lab 4
Laboratory 5 - Pathogens of the Respiratory Tract
40
Aims
41
Biofilm Formation Assay
42
Identification of mycobacteria
43
Differential stains - acid-fast staining
44
Lab 5 Protocols
45
Lab 5 - Data Analysis
46
Additional Resources/Further Reading for Lab 5
Appendix
47
Basic Lab Calculations
48
Media Used in these Labs
Appendix
47
Basic Lab Calculations
47
Basic Lab Calculations
Appendix
48
Media Used in these Labs